The world’s first semi-automated colony counter provides accurate results of Legionella pneumophilia and Pseudomonas aeruginosa in environmental samples in record time


Diamidex have developed and produced the world’s first semi-automated microcolony counter. With its unparalleled optical resolution and state-of-the-art software that utilizes machine learning capabilities, the MICA Fluorescence Counter detects and counts microorganisms in their early microcolony stage.

This instrument allows operators to count microcolonies on a membrane. The MICA counter has an optical system that takes very high resolution snapshots of each membrane; these are then analyzed by the counter software to accurately determine the number of microorganisms on each membrane.

MICA is a user-friendly, turnkey system in which the end user is led, one step at a time, through a protocol for the targeted microorganism. Automated counting significantly reduces the amount of uninterpretable results compared to traditional culture methods, but faster turnaround time.

This avant-garde but user-friendly device will not force you to change your habits; you can automatically count Pseudomonas aeruginosa grown on your usual CN agar plate, in CFU, but much faster. You simply follow the usual NF EN ISO 16266 procedure and there is no need to change or add reagents. Incubation is reduced from 44 to 24 hours – and using MICA to count P. aeruginosa at its microcolony stage means CFU data is ready in just 24 hours, instead of the 44 hours you need with a manual counting.

Legionella pneumophila is a ubiquitous bacterium; it grows in its natural habitat, which is warm water, and adapts to other environments with similar characteristics, including the human body, which has the ideal temperature for the growth of this pathogenic bacterium. When inhaled, Legionella pneumophila can enter the lungs to replicate until it causes the potentially fatal Legionnaires’ disease.

Thanks to Diamidex technology, it is now possible to detect and enumerate Legionella pneumophila after only 48 hours of incubation on a Petri dish, compared to 10 days with the standard method. Only culturable Legionella pneumophila are counted from all serogroups, so there are fewer uninterpretable and more accurate results; all Legionella pneumophila micro-colonies present are counted rather than a few representative ones. This technology also offers a much more economical way to test Legionella pneumophila than conventional methods.


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